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This is just a quick cheatsheet of PVS tweaks we make.  All of these tweaks are essential to actually being able to capture a vdisk with PVS 6.1.

PVS Server Tweaks 

• HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Services\TCPIP\Parameters\

               Key: “DisableTaskOffload” (dword)

               Value: “1”

• Disable IPv6
• Disable Network Monitor Driver
• Set Stream and Soap services recovery actions to Restart
• Set permissions on the Store folder to grant Network Service full permission and to the child folders if any

Target Device Tweaks

On target devices (XenApp servers or virtual desktops), add the following entries to the registry:

• HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Services\TCPIP\Parameters\

             Key: “DisableTaskOffload” (dword)

             Value: “1”

• Remove Ghost Nics: http://support.citrix.com/article/CTX133188
• Disable IPv6
• Disable Network Monitor Driver
• Install all required MS patches (MS433809 for 64bit or MS421114 for 32bit)
• Install latest PVS client based on your requirements

Another method to clone can be found here: http://blogs.citrix.com/2012/12/05/deploy-xenapp-6-5-using-pvs-6-1-part-1/